Aucun titre de diapositive

Aucun titre de diapositive

Liverpool, 17-21 February 2003 INRA, Avignon, France V. Chovelon, research scientist JP Leroux, research technician F. Mirrione, temporary agent (1.5 month) Somatic embryogenesis, genetic characterisation plant regeneration acclimatisation and field evaluation sanitary aspects meristem culture on selected clones production of virus-free bulbs Embryogenesis Variety In vitro culture - cal - ECS - bulbif. essay Acclimatisation 1st field evaluation - field notations - cytometry - AFLP - biochemistry 2nd field evaluation RR MES MOL PRI done (1) done (1) done (2)

done (2) done (2) done (3) done (2) done (2) done (2) done (2) done (2) done (3) done (2) done (2) done (2) done (3) done (2) done (2) done (3) done (3) done (3) done (3) to be done (4) done (3) done (3) done (3) to be done (4) - Sanitary aspects Virus elimination - meristem culture - acclimatisation - virus control - field culture

VIII.1.96 VII.81.97 done (1) done (1) done (2) done (2) done (2) done (2) done (3) done (3) Production of virus-free bulbs done (1) (2) - Rouge Reunion done (1) (2) (3) (4) - Messidrme done (1) (2) (3) (4) - Morasol done (1) (2) (3) (4) - Printanor N DP1 DP5 Title virus-free bulbs materiel for introduction in vitro Evidence of a somatic embryogensis process and plant regeneration and acclimatization in garlic (Allium sativum L.) L. FEREOL*, V. done (1) (2) (3) (4) done (3) CHOVELON**, S. CAUSSE*, N. MICHAUXFERRIERE*, R. KAHANE*. Plant Cell Report (2002) 21:197-203. DP13 Establishment of embryogenic cell suspensions cultures of garlic (Allium sativum L.) and plant regeneration. L. FEREOL*, V. CHOVELON**, S.

CAUSSE*, D. TRIAIRE, N. MICHAUX-FERRIERE*, R. KAHANE*. DP21 Embryogenic cell suspension cultures of garlic (Allium sativum L.) as method for mass propagation and convenient material for genetic improvment. L. FEREOL*, V. CHOVELON**, S. CAUSSE*, R. KAHANE* delayed writing in progress (4) delayed writing in progress (4) plants from callus and cell suspensions In vitro culture (1) acclimatisation (2) AFLP analyses flow cytometry phenotypic notations - 1st field evaluation at Reunion Island by P8 (3) Plants origin RR ecs (T60 ) RR ecs (B) RR ecal (T60) RR ecal (B) Vegetative multiplic.

Number cloves planted plants in vegetation % mean Height Mean in cm Leaves number mean Homogeneity (1 to 5) mean Vigour (1 to 5) mean Colour of plants 183 72 287 70 122

72 93 70 89 81 25.02 +/- 1.9 24.362 +/- 1.44 23.27 +/- 1.96 29.24 +/- 3.13 39.29 +/- 3.49 6.09 +/- 0.31 6.15 +/- 0.33 5.7 +/- 0.47 6.5 +/- 0.61 6.61 +/- 0.45 3.45 +/- 0.26 3.01 +/- 0.25 3.31 +/- 0.25

3.53 +/- 0.35 4.2 +/- 0.2 2.95 +/- 0.23 2.72 +/- 0.19 2.87 +/- 0.31 3.39 +/- 0.34 4.25 +/- 0.21 Greenyellow Greenyellow Greenyellow Greenyellow Greenyellow plants from callus or cell suspensions In vitro culture (2) - regeneration - bulb maturation Acclimatisation (2) 1st field culture (3) (4) 166 Mes cal; 145 Mol cal; 125 Mol ecs; 153 Mes ecs 2nd field culture (4) - 282 Messidrme cal - 143 Morasol cal - 145 Morasol ecs 1st field evaluation

notations (plant development, height, leaves colour, bulbs shape and weight) plody level variety Plants tested Plody level 2X 4X RReunion-cal 120 120 0 RReunion-ECS Messidrme-cal Morasol-cal Morasol-ECS 120 100 100 100 120 90 97 96 0

10 3 4 1st field evaluation sulphur compounds concentration (HPLC) 70 Morasol and Messidrme 60 Qb alliine Qb allicine Qb GLUAlCS Qb GLUPeCS 50 40 30 20 10 alliin Rouge Reunion_lot1 0 allicin 60 50 MORcal MORecs MEScal

MORcee GLUAlCS isoGLUAlCS 40 30 20 10 0 CAL SC VG Rouge Reunion MEScee 1st field evaluation sulphur compounds proportion (HPLC) 60,00 Morasol and Messidrme alliin 50,00 allicin GLUAlCS 40,00 IsoGLUAlCS

30,00 20,00 AlCSO lot 1 TiAl2 50GLUAlCS GLUPeCS 40 10,00 0,00 MORcal MORecs 30 20 Rouge Reunion 10 0 CAL SC VG MEScal MORcee MEScee

plants from callus Plants development Vitroplants Rooted plants In vitro (2002) acclimat. (April 02) greenhouse (Oct 2002) 348 plants 348 plants Plants in vegetation 117 plants Bulbs at maturity 126 bulbs Plants in vegetation Bulbs at maturity field cult. (2003) 222 plants production of virus-free plants Messidrme, Morasol, Printanor 300 plants / var. (Nov-Dec 02)

virological control all the plants were potyvirus-free some one contained latent viruses Messidrme: 100% GCLV Morasol: 83% MbFV Printanor: 100% MbFV virus elimination by meristem culture clone In vitro acclimatisation Greenhouse Tunnel VIII.1.96 30 (June 01) 10 (June 01) 19 (June 02) 30 (June 02) 30 (Sept 01) 10 (Sept 01) 19 (Sept 02) 30 (Sept02)

37 plants (febr. 02) 10 plants (febr. 02) 19 plants (febr. 03) 30 plants (febr. 03) 37 bulbs (20 to 75g) 10 bulbs (12 to 80g) VII.81.97 3 4 Embryogenesis, genetic characterisation Rouge Reunion 2nd field evaluation (P8), biochemistry (P5) Messidrme, Morasol and Printanor from callus 2nd field culture and evaluation (P9) flow cytometry (P8), AFLP (P1), biochemistry (P5) Messidrme, Morasol and Printanor from cell suspension 1st field evaluation flow cytometry (P8), AFLP (P1), biochemistry (P5) Sanitary aspects production of virus-free bulbs (P9)

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