DNA Extraction from wheat germ - Salk Institute for ...

DNA Extraction from wheat germ - Salk Institute for ...

DNA Extraction from wheat germ Protocol Salk Institute Mobile Lab Step #1 Obtain a numbered 50 ml conical vial with 10 ml of water.

Write your number here:________ Salk Institute Mobile Lab Step #2 To the tube with 10 ml water add 1g (1 pinch) of raw wheat germ. The wheat germ should settle to fill 1/2 the

cone Salk Institute Mobile Lab Step #3 Cap the tube and vigorously shake to mix for about 30 seconds. What do you think the shaking is

doing to the wheat cells? Salk Institute Mobile Lab After shaking - let the cell walls settle to the bottom Whats in the cloudy solution? Where is the DNA

at this point? Salk Institute Mobile Lab Step #4 Using a transfer pipet add 3 ml of 25% dish soap to the tube with water and wheat germ. Salk Institute Mobile Lab

Steps #5-6 Cap the tube and slowly invert several times to mix (DO NOT SHAKE). Loosen the cap slightly before placing tube in the hot water bath. Heat at 65C for 5

minutes. Salk Institute Mobile Lab What do you think the soap will do? How will the hot water bath help? Bonus question: Why does washing your hands with soap and hot water kill bacteria on your skin? Salk Institute Mobile Lab

Step #7 While the tubes are heating label a 1.7 ml eppendorf tube with your initials. Carefully open the epi tube and place it into the rack Salk Institute Mobile Lab

Steps #8-9 Before removing your tube from the 65C water fill a transfer pipet with 3ml of alcohol. Bring the 50 ml conical tube back to your table and as soon as possible add the 3ml of alcohol to the tube.

Salk Institute Mobile Lab Step #10 Watch the DNA rise out of the green soap layer and into the alcohol layer. What color is DNA?? What color do you think YOUR DNA is? Why?

Salk Institute Mobile Lab Step #11 After waiting a few moments to allow the DNA to precipitate remove the DNA using an inoculation loop and place in your eppendorf tube. Try to avoid any wheat germ

debris that is present. Salk Institute Mobile Lab Step #11 cont. Spinning the loop will help get the DNA off Make sure to close the epi tube securely (you should hear it click)

Salk Institute Mobile Lab Congratulations! You have just done something that scientists do everyday in the lab! Your DNA will last you for years its very stable when stored in isopropanol SOME RULES: Your DNA tube should be put away during school - other teachers will

confiscate it if you disturb their class! You must show your parents at home your DNA and tell them what you learned! Salk Institute Mobile Lab Review: We extracted DNA from wheat germ And discovered that the color of DNA is

WHITE! No matter what organism it comes from, DNA in a tube looks the same! Salk Institute Mobile Lab We ALL have white DNA!! Salk Institute Mobile Lab

Recently Viewed Presentations

  • OAMA 2017 State CIP  Update March 6, 2017

    OAMA 2017 State CIP Update March 6, 2017

    OAMA 2017 State CIP - Update March 6, 2017 JPCs held October 2016 FAA CIP Letters - Spring 2017 Update CIPs Summer/Fall ready for JPCs Mid-Fall 2017 to look at '19-'23 SCIP - Update Use Up "Carry Over" NPEs Transfer...
  • CAMPBELL BIOLOGY TENTH EDITION Reece  Urry  Cain  Wasserman

    CAMPBELL BIOLOGY TENTH EDITION Reece Urry Cain Wasserman

    the result of cambial initials alternately forming sieve-tube elements and tracheids. the annual scars formed by the death of the periderm. a result of changes in diameter of tracheids depending on available water and favorable temperature. Answer: E
  • Tminus10 - MIDWEST LENDERS

    Tminus10 - MIDWEST LENDERS

    The capitalization rate support must describe and analyze how the sale's risk and potential for income fluctuations impacts the capitalization rate. ... A tool to help determine the risk level of a particular project and flags items that may need...
  • ¡Verb Mania!

    ¡Verb Mania!

    ¡Verb Mania! CHARTS! CONJUGATE-ar verb endings. o. amos. as. áis. a. an
  • Trait Theories - UMass D

    Trait Theories - UMass D

    Trait Theories Basic Assumptions and Central Points behavior determined by stable generalized traits basic qualities that exist within a person and express themselves across situations goal of trait psychology: determine those trait dimensions determine where people stand relative to others...
  • Origin of The Martian Hemispheric Dichotomy: The case for ...

    Origin of The Martian Hemispheric Dichotomy: The case for ...

    Origin of The Martian Hemispheric Dichotomy: The Case for Impacts David Galvan ... (A.D. Fortes, 1999) Exogenic Processes Impacts! Anatomy of an Impact Observing remnants of Impacts Early Suggestions: Giant Impact Wilhelms and Squyres (1984) Suggest a single giant impact...
  • Tilda Goldberg: Lessons for Today from a Social

    Tilda Goldberg: Lessons for Today from a Social

    Professional careers, at a time when this was highly unusual for women ... April 1933 Law for the Restoration of a Professional Civil Service - Ayrian Paragraph. ... London School of Economics. 1933-35 LSE - Certificate in Social Science and...
  • Welcome to Ottawa's First Showing of

    Welcome to Ottawa's First Showing of

    Title: Welcome to Ottawa's First Showing of Author: Yves Last modified by: Grys Created Date: 11/29/2004 7:33:40 PM Document presentation format