LA GALERIE API 20E - Free

LA GALERIE API 20E - Free

LA GALERIE API 20E 1- Prsentation de la galerie Galerie de 20 microtubes prts lemploi permettant de raliser 23 tests biochimiques afin didentifier des bacilles Gram appartenant la famille des ENTEROBACTERIACEAE. cupule microtube contenant le milieu dshydrat La suspension bactrienne introduite dans le tube dissout les substrats dshydrat 2- Prparation de linoculum 1 seule colonie isolement

Prlvement dune souche pure 5 mL dED strile Suspension dopacit 0,5 sur lchelle de Mac Farland Souche pure sur GNi 3- Ensemencement de la galerie API 20 E Introduire la suspension bactrienne dans chaque tube laide dune pipette Pasteur strile, pointe appuye lintrieur et sur le ct pour viter la formation de bulles Pour certains caractres:

Remplir de suspension le tube et la cupule CIT, VP, GEL Remplir le tube de suspension et recouvrir dhuile de paraffine ADH, LDC, ODC, H2S, URE 4- Lecture de la galerie API 20 E Les 10 premiers tests Tests ngatifs Tests positifs Les 10 derniers tests

Tests ngatifs Tests positifs NR + 5- Identification de la souche Rsultats de la galerie: - 5 2 1

5 7 7 3 ultats reports sur la fiche didentification Code n: 5 215 773 (55) Se rfrer au catalogue pour identifier la souche laide du code +

+ 5 + + 5

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