Comparison of the Cytotoxic Effects of Silver Sulfide

Comparison of the Cytotoxic Effects of Silver Sulfide

Comparison of the Cytotoxic Effects of Silver Sulfide Quantum Dots Coated with 2-mercaptopropionic acid and meso-2,3dimercaptosuccinic acid in V79 Cells. Deniz zkan Vardar1, Ibrahim Hocaolu2, Havva Funda Yac Acar3, Nuren Baaran4 Hitit University, Sungurlu Vocational High School, Health Programs, Sungurlu, orum, Turkey 2 Koc University, Materials Science and Engineering, Istanbul, Turkey 3 Koc University, Department of Chemistry, Istanbul, Turkey 4 Hacettepe University, Faculty of Pharmacy, Department of Toxicology, Ankara, Turkey 1 Deniz ZKAN Toxicology & Applied Pharmacology VARDAR 20-22 October2012

1 Over the past twenty years, the nanotechnology industry is rapidly growing. With this dramatic expansion the safety evaluation and the risk management of nanomaterials are the important requirements. Toxicology & Applied Pharmacology Deniz ZKAN VARDAR

20-22 October 2012 2 Due to the distinct physico-chemical proterties of many nanomaterials, their possible toxicity may differ from the bulk material of similar chemical nature. Toxicology & Applied Pharmacology Deniz ZKAN VARDAR 20-22 October 2012

3 Nanomaterials may genotoxic properties have unpredictable o Because of their small size and high surface area and other physico-chemical features such as charged surfaces. Toxicology & Applied Pharmacology Deniz ZKAN VARDAR 20-22 October 2012

3 Nanomaterials may genotoxic properties have unpredictable o Because of their small size and high surface area and other physico-chemical features such as charged surfaces. Toxicology & Applied Pharmacology Deniz ZKAN VARDAR 20-22 October 2012

4 These materials can entry into the body via inhalation, dermal or oral routes. So nanomaterials can promote cell damage with direct and indirect mechanisms Toxicology & Applied Pharmacology Deniz ZKAN VARDAR 20-22 October 2012 5 Quantum dots have semiconductor

nanocrystals (size ranges between 2 to 6 nm) that have unique spectral properties. The semiconductor particle (named core) is usually coated by a layer of another semiconductor material (named shell) which in general has a greater band gap than the band gap of the core rendering excellent optical properties. Toxicology & Applied Pharmacology Deniz ZKAN VARDAR 20-22 October 2012

6 Quantum dots have optical and electrical properties currently applied in biomedical imaging and electronics industries. Applications of QDs have been mostly studied on mammalian cells, such as biomedical applications that multifunctional drug delivery, and photodynamic therapy. Toxicology & Applied Pharmacology Deniz ZKAN VARDAR

20-22 October 2012 7 Deep understanding of their effects in the cellular environment and their cytotoxic effects are still lacking. Toxicology & Applied Pharmacology Deniz ZKAN VARDAR 20-22 October 2012 8 Cellular responses and their effects were determined by the cytotoxicity assays are based on different modes of detection like

neutral red uptake and MTT assays. Neutral red assay has been used for the identification of vital cells in cultures. This assay quantifies the number of viable, uninjured cells after their exposure to toxicants; It is based on the uptake and subsequent lysosomal accumulation of the supravital dye, neutral red. Toxicology & Applied Pharmacology Deniz ZKAN VARDAR 20-22 October 2012 9 Another assay as a colorimetric assays is the metabolic activity of viable cells. Tetrazolium salts are reduced only by metabolically active cells.

Thus, 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide (MTT) can be reduced to a blue colored formazan and the amount of formazan can be correlated to the amount of viable cells. Toxicology & Applied Pharmacology Deniz ZKAN VARDAR 20-22 October 2012 10 In this study; the aim was to evaluate the cytotoxic potential of Silver sulfide quantum dots coated with 2mercaptopropionic acid (2MPA)and meso-2,3dimercapto succinic acid (DMSA). For this purpose Chinese Hamster Lung Fibroblast cell lines (V79) were treated with quantum dots in the concentration range of 5-2000 g/mL for 24 h. g/mL for 24 h.

Toxicology & Applied Pharmacology Deniz ZKAN VARDAR 20-22 October 2012 Materials and Methods 11 Materials Silver sulfide quantum dots coated with 2mercaptopropionic acid (2MPA)and meso-2,3dimercapto succinic acid (DMSA) were synthesized at the Department of Chemistry, Faculty of Science, Ko University. QDs were characterized in terms of size and size distribution. Toxicology & Applied Pharmacology

Deniz ZKAN VARDAR 20-22 October 2012 12 Methods Neutral Red Assay; V79 cells were seeded in flasks in Dulbeccos modified Eagles medium (DMEM). After 24 h incubation, cells were treated with quantum dots in the concentration range of 5-2000 g/mL for 24 h. g/mL for 24 h The cells were washed twice with PBS and incubated for an additional 3 h in the medium supplemented with NR After the medium was discarded, the cells were rinsed three times with warm

PBS to remove the nonincorporated excess dye and 200 l of destain solution was added to each well to fix the cells and bring the NR into solution. The plates were shaken for 20 min, and the absorbance of the solution in each well was measured in a microplate reader at 540 nm and compared with wells containing untreated cells. Toxicology & Applied Deniz ZKAN Pharmacology VARDAR 20-22 October 2012

12 Methods The cytotoxicity of QDs quantitatively evaluated by assay. was MTT Cells seeded in 96-well plates were separately treated with different concentrations of quantum dots for 24 h. 10 microliters of MTT (5 mg/mL) was added to each well and incubated for another 4 h at 37C. Next, 100 g/mL for 24 h. L DMSO was added to each well, and the optical density at

540 nm was recorded on a microplate reader. Toxicology & Applied Pharmacology Deniz ZKAN VARDAR 20-22 October 2012 13 Results The effects of the Ag2S-(2mercaptopropionic acid) and Ag2S-(2-meso-2,3dimercaptosuccinic acid) on cell viability at different concentrations were determined by NRU assay

after 24 h treatment. Data were expressed as the percentage of the viable cells to non-treated control cells, and represent three independent experiments. Toxicology & Applied Pharmacology Deniz ZKAN VARDAR 20-22 October 2012 14 Results The effects of the Ag2S-(2mercaptopropionic acid) and Ag2S-(2-meso-2,3dimercaptosuccinic acid) on cell viability at different

concentrations were determined by MTT assay after 24 h treatment. Data were expressed as the percentage of the viable cells to non-treated control cells, and represent three independent experiments. 15 Conclusion The present study was aimed in vitro assessment of the cytotoxic potential of these two kinds of QDs on V79 cell line. In summary, the results manifested that when modified with different QDs still possessed excellent biocompatibility and low cytotoxicity to cells, which may make them more promising in bioimaging and other biomedical applications. Toxicology & Applied

Pharmacology Deniz ZKAN VARDAR 20-22 October 2012 Prof. Dr. Nuren Baaran Hacettepe University Department of Toxicology Assoc. Prof. Funda Yac Acar Ko University, Department of Chemistry Dr. brahim Hacolu Koc University, Materials Science and Engineering

Toxicology & Applied Pharmacology Deniz ZKAN VARDAR 20-22 October 2012 THANK YOU FOR YOUR ATTENTION Toxicology & Applied Pharmacology Deniz ZKAN VARDAR 20-22 October 2012

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