An Introductory Information about Optical Tweezers Mustafa Yorulmaz

An Introductory Information about Optical Tweezers Mustafa Yorulmaz Ko University, Material Science and Engineering Outline History of optical tweezers Basics of optical tweezers Physics beyond optical trapping Types of optical tweezers Possible uses and research areas A typical setup of optical tweezers Illustrative examples from our laboratory

February 20, 2008 Ko University, Material Science a nd Engineering 2 History of Optical Tweezers 1970 - Optical Tweezers: A. Ashkin (*) The technique of trapping was first applied in 1984 at Bell Laboratories In 1986, they showed a demo of optical tweezers They showed the damagefree manipulation on cells using an infrared laser in 1987. (*) http://www1.bell-labs.com/org/physicalsciences/timeline/1970_ashkin_pop.html

February 20, 2008 Ko University, Material Science a nd Engineering 3 Basics of Optical Tweezers Optical Tweezers are examples of optical trapping which is a subfield of laser physics. They are used to trap and manipulate particles (in range from m to nm) and also to measure the forces (in range from 1 to 100 pN) on these particles A strongly focused laser beam is used for trapping the particles. The particles that are of interest has to show dielectric property. * http://www.physics.uq.edu.au/lp/tweezers/papers/nieminen2001jqsrt70/

February 20, 2008 Ko University, Material Science a nd Engineering 4 Physics Beyond Optical Trapping Trapping occurs due to radiation pressure of laser which results by the momentum change of light. There are two forces that let the particle to be hold in trap: Scattering force: due to reflection of light (to push object)

Gradient force: due to refraction of light (to pull object) nparticle>nmedium so that Fgradient>Fscattering Using high NA objectives and high power lasers maximizes the trapping force. February 20, 2008 Schematic diagram showing the force on a dielectric sphere due to both reflection and refraction of two rays of light. Ko University, Material Science a nd Engineering 5 Types of Optical Tweezers

Holographic Optical Tweezers Single beam optical tweezers Dual beam optical tweezers Experimental setup for our aerosol holographic tweezers http://www.the-scientist.com/article/display/15545/ http://www.ffn.ub.es/ritort/research.html http://www.dundee.ac.uk/elecengphysics/ tweezers/Research/ aerosols_HOT.htm http://people.sabanciuniv.edu/meric/projects/twz1.jpg February 20, 2008 Ko University, Material Science a nd Engineering 6

Possible uses and research areas By using optical tweezers, it is possible to Optical tweezer for force measurements study and manipulate particles like atoms, molecules and small dielectric spheres (in range from m to nm). make force measurements of biological objects in piconewton range. So, optical tweezers has opened up several important new areas of study in biophysics Dielectric objects are attracted to the center of the beam, slightly above the beam waist, as described in the text. The force applied on the object

depends linearly on its displacement from the trap center just as with a simple spring system. http://www.answers.com/topic/optical-tweezers?cat=techn In the lasest research; this technique has been used for biological investigations involving cells cutting and ablating biological objects (cell fusion and DNA cutting) force measurements of cell structures and DNA coiling elasticity measurements of DNA February 20, 2008 Ko University, Material Science a nd Engineering 7 A Typical Setup of Optical Tweezers f2

M1 f1 Collimation Nd: YAG Laser (1064nm) Illumination Microscope f3 f4 Telesentric M3 M2 O1 f5 CCD

M4 February 20, 2008 Ko University, Material Science a nd Engineering Computer 8 Illustrative Examples From Our Laboratory: Manipulation of Microspheres Thanks to Ahmet Faruk Cokun February 20, 2008 Ko University, Material Science a nd Engineering 9 Illustrative Examples From Our

Laboratory: Manipulation of Vibrio Cholerae Thanks to Ahmet Faruk Cokun February 20, 2008 Ko University, Material Science a nd Engineering 10 Thanks.. Questions? February 20, 2008 Ko University, Material Science a nd Engineering 11

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