St. Helena Rootstock Survey Trial Dr. Jude Grosser

St. Helena Rootstock Survey Trial Dr. Jude Grosser

St. Helena Rootstock Survey Trial Dr. Jude Grosser - Dr. Fred Gmitter - Dr. Bill Castle Orie N. Lee and Lee Family Groves October 8, 2019 CREC Citrus Plant Improvement St. Helena Rootstock Survey Trial - Description HISTORY: The St. Helena Project was spear-headed by the late Hall of Fame Citrus Grower/Researcher Orie N. Lee. Mr. Lee made significant financial and intellectual contributions to the project. He found and purchased the property (19.9 acres on St. Helena Rd., east of Dundee), had the old trees cleared, fixed the well twice, provided a back-up diesel generator, and had a protective structure built over the well pump. Since this time (2008), the Lee Family has been leasing the property to the Citrus Research and Education Foundation (CREF) for $1/year. Profits from harvested fruit have gone 100% back to the CREF to support research grove care. The original idea for the project was to test new sweet orange selections that had potential to improve juice products. However, as HLB spread across the state, the objective changed to evaluating as many new rootstock candidates as possible to identify those that could impart HLB tolerance. The UF/CREC Citrus Improvement Team (Grosser, Gmitter and Castle) then gathered the available rootstocks (propagations from both seed and cuttings) including many diploid and tetraploid selections from the UF/CREC breeding program along with selections from the Sicilian (Reforgio-Recuperato) and Argentinian (Foguet) rootstock breeding programs. Vernia and Valquarius, mid-season processing oranges that generally can be harvested beginning mid-January with Valencia quality, were chosen as the scions for the project. CREC Citrus Plant Improvement St. Helena Rootstock Survey Trial - Description PLANTING AND TREE CARE: The first set of trees was planted in 2008 [about 12 acres] in 4-tree rectangular plots. Trees were planted in one of 3 sections with a different spacing in each section: [1] 9 x 20 [for dwarf-sized trees]; [2] 12 x 20; and [3] 15 x 25 ft. Rootstocks were chosen for a particular section based on a predicted/expected size. There were uneven numbers of 4-tree plots for each rootstock selection. HLB was first observed in the trial 6 months after the initial planting. The trial has had strong HLB pressure during its entire life as there was an unsprayed block of K-earlies adjacent to the trial block on the east side and, later, a top-worked block on the south side that was a major psyllid attractant. A second major set of trees was planted in 2010. There were two subsequent plantings, but they were devastated by freezes primarily because the irrigation well volume was not sufficient to provide cold-protection via microjets to the entire trial. As a result, CRDF funded the installation of a larger well that now provides cold protection for the entire trial. Cultural care of the trial is provided by the CREC Grove Care Team managed by Troy Gainey. CREC Citrus Plant Improvement St. Helena Rootstock Survey Trial - Description NUTRITION: The nutrition/irrigation program was designed to minimize any stress on the trees and, thereby, allow maximum expression of HLB tolerance. The trees at the beginning were fertilized with a Harrells nursery mix of controlled release fertilizer [CRF] applied about every 6 months. Initially, trees were irrigated 3 times per week, but that schedule was later changed to a daily irrigation regime with shorter application intervals. In cooperation with Harrells, who supplied their products at no cost, the CRF was modified to enhance the micronutrient content. The first change was to use a CRF mix that contained calcium nitrate and boron (not included in the nursery mix). Next, the revised mix contained elevated micronutrients, some provided in the TigerSul form. In January 2018, the CRF mix was modified to increase the nutrients again, especially magnesium and manganese (see the 14-3-11 label). A noticeable improvement in tree appearance was observed. Over the 2017-18 and 2018-19 seasons, the trees received supplemental soluble dry fertilizer via broadcasting. CREC Citrus Plant Improvement St. Helena Rootstock Survey Trial Description DATA COLLECTION: Yield has been measured annually in boxes/tree and fruit quality samples of about 50 fruit/plot have been collected since 2011. An effort was made to obtain data from a minimum of one 4-tree plot for each scion/rootstock combination each season and from two plots per combination when possible. Tree canopy size was also measured and used to predict the optimum tree spacing for each combination. Measured yield and fruit quality data were combined to estimate the pounds-solids that could be produced for each scion/rootstock combination if trees were planted at their optimum spacing. COMMENTS: HLB quickly moved into this trial and initially appeared among the trees on the standard commercial rootstocks. The older, established trees have been 100% infected for several years. The 2017-18 season was below average with low yields and reduced juice quality across the entire trial possibly because of Hurricane Irma and a significant delay in one of the CRF applications. However, in the 2018-19 season there was a 62% increase in yield and a 20% increase in juice quality across the entire trial. Although many rootstocks showed a good recovery in the 2018-19 season, there are a few rootstocks that have performed well during the entire life of the trial. CREC Citrus Plant Improvement St. Helena Rootstock Survey Trial Location: Dundee, Polk county Scion: Vernia & Valquarius Rootstocks: large collection of new diploid and tetraploid rootstocks Date Planted: 2008 & 2010 Design: According to the number of trees available Replications: variable per rootstock Plot size: 4-tree rectangular plots Spacing: 9 x 20; 12 x 20; 15 x 25 ft. Data: Yield and juice quality: 9 seasons, 2010-11 to 2018-19 Tree size: 2019 CREC Citrus Plant Improvement Table 1. St. Helena Rootstock Survey Trial Rootstock parentage and 2018-19 survival of trees planted in 2008, sorted by survival, % Scion[s] Valq Valq Valq Valq Valq Valq Valq/Vernia Valq/Vernia Valq/Vernia Valq/Vernia Valq/Vernia Valq/Vernia Valq/Vernia Valq/Vernia Valq Vernia Valq/Vernia Valq/Vernia Valq Valq/Vernia Rootstock FG 1702 FG 1707 FG 1709 FG 1731 [UFR-13] FG 1733 [UFR-14] FG 1792 Volk Orange 19 [UFR 4] Kuharske Orange 1804 White 4 [UFR 5] Orange 21 Orange 16 Orange 4 [UFR 2] 681G26F4P2 Yellow 1800 Cleo+CZO Orange 3 [UFR 1] 681G26F2P12 Aqua 1803 Rootstock Parentage TBD* TBD TBD putative C. ichangensis/Poncirus hybrid

putative pummelo/mandarin hybrid TBD Volkamer lemon [Nova + HBPummelo] x [Cleopatra + Argentine trifoliate orange] Carrizo open-pollinated zygotic Cleopatra x trifoliate orange (Arg.) [Nova + HBPummelo] x [Succari + Argentine trifoliate orange] [Nova + HBPummelo] x [Cleopatra + Argentine trifoliate orange] [Nova + HBPummelo] x [Cleopatra + Argentine trifoliate orange] [Nova + HBPummelo] x [Cleopatra + Argentine trifoliate orange] C. latipes x trifoliate orange (Sic.) Grapefruit x trifoliate orange (Arg.) Cleopatra + Carrizo [Nova + HBPummelo] x [Cleopatra + Argentine trifoliate orange] C. latipes x trifoliate orange (Sic.) Cleopatra x trifoliate orange (Arg.) Ploidy No. trees planted No. survived Survival**, % 2x 2x 2x 2x 2x 2x 2x 4x 2x 2x 4x 4x 4x 4x 2x 2x 4x 4x 2x 4x 2 3 4 4 5 2 20 124 22 19 74 46 25 50 12 12 164 62 10 19 2 3 4 4 5 2 20 120 21 18 70 43 23 46 11 11 150 56 9 17 100 100 100 100 100 100 100 97 95 95 95 93 92 92 92 92 91 90 90 89 (*) To be determined (**) Caveat: Many trees of both scions that were lost during the trial were removed early only because of poor condition at planting. Thus, the tree survival results may be somewhat unrepresentative of true performance. CREC Citrus Plant Improvement Table 1 (contd). St. Helena Rootstock Survey Trial Rootstock parentage and 2018-19 survival of trees planted in 2008, sorted by survival, % Scion[s] Rootstock Rootstock Parentage Ploidy No. trees planted No. survived Survival**, % Valq/Vernia Valq/Vernia Valq/Vernia Valq/Vernia Vernia Valq/Vernia Valq/Vernia Valq/Vernia Valq Valq/Vernia Valq/Vernia

Valq/Vernia Valq/Vernia Valq/Vernia Valq/Vernia Valq/Vernia Valq Valq/Vernia Valq/Vernia Orange 2 Wgft+50-7 Orange 15 [UFR 3] SO+CZO Orange 1 Orange 18 Chang+50-7 [UFR 6] Orange 14 681G26F4P6 Blue 1 Chang+Bent Cleo SO+50-7 Orange 13 White 1805 Amb+HBJL-2B 681G26F6P20 Purple 4 Rough lemon [Nova + HBPummelo] x [Cleopatra + Argentine trifoliate orange] White grapefruit + trifoliate orange [Nova + HBPummelo] x [Cleopatra + Argentine trifoliate orange] Sour orange + Carrizo [Nova + HBPummelo] x [Cleopatra + Argentine trifoliate orange] [Nova + HBPummelo] x [Cleopatra + Argentine trifoliate orange] Changsha mandarin + trifoliate orange 50-7 [Nova + HBPummelo] x [Cleopatra + Argentine trifoliate orange] C. latipes x trifoliate orange (Sic.) [Nova + HBPummelo] x [sour orange + Palestine sweet lime] Changsha mandarin + Benton citrange Cleopatra mandarin Sour orange + trifoliate orange 50-7 [Nova + HBPummelo] x [Cleopatra + Argentine trifoliate orange] Ruby blood orange x Barnes trifoliate orange (Arg.) C. amblycarpa + HBJL-2B pummelo C. latipes x trifoliate orange (Sic.) [Nova + HBPummelo] x [Cleopatra + sour orange] Rough lemon 4x 4x 4x 4x 4x 4x 4x 4x 2x 2x 4x 2x 4x 4x 2x 4x 2x 2x 2x 55 89 43 267 25 25 56 46 13 60 18 18 41 52 20 28 19 77 17 49 79 38 235 22 22 49 40 11 50 15 15 34 42 16 22 15 59 13 89 89 88 88 88 88 88 87 85 83 83 83 83 81 80 79 79 77 76 Valq/Vernia White 1801 Ruby blood orange x Barnes trifoliate orange (Arg.) 2x 12 9 75 (*) To be determined (**) Caveat: Many trees of both scions that were lost during the trial were removed early only because of poor condition at planting. Thus, the tree survival results may be

somewhat unrepresentative of true performance. CREC Citrus Plant Improvement Table 1 (contd). St. Helena Rootstock Survey Trial Rootstock parentage and 2018-19 survival of trees planted in 2008, sorted by survival, % Scion[s] Rootstock Rootstock Parentage Ploidy No. trees planted No. survived Survival**, % Valq/Vernia Green 7 [Nova + HBPummelo] x [sour orange + Carrizo] 4x 47 35 74 Valq/Vernia MG-11 Hirado Buntan pummelo - zygotic from open pollination 2x 39 28 72 Valq/Vernia Swingle White grapefruit x trifoliate orange 2x 22 15 68 Valq/Vernia Blue 9 [Nova + HBPummelo] x [sour orange + Palestine sweet lime] 2x 35 23 66 Valq/Vernia Pink 1802 Cleopatra x Swingle citrumelo (Arg.) 2x 19 12 63 Valq/Vernia Blue 4 [Nova + HBPummelo] x [sour orange + Palestine sweet lime] 2x 45 27 60 Valq FG 1793 TBD* 2x 5 3 60 Valq/Vernia Blue 2 [Nova + HBPummelo] x [sour orange + Palestine sweet lime] 2x 32 19 59 Valq/Vernia Purple 2 [Nova + HBPummelo] x [Cleopatra + sour orange] 4x 22 12

55 Valq SO+RPxSH99-5 Sour orange + rangpur, open pollinated 4x 17 8 47 Valq/Vernia Amb+HBJL-1 C. amblycarpa + HBJL-1 pummelo 4x 16 7 44 Valq/Vernia 69LTXamF14P37 C. latipes x sour orange (Sic.) 2x 7 3 43 Valq/Vernia Blue 3 [Nova + HBPummelo] x [sour orange + Palestine sweet lime] 2x 53 19 36 (*) To be determined (**) Caveat: Many trees of both scions that were lost during the trial were removed early only because of poor condition at planting. Thus, the tree survival results may be somewhat unrepresentative of true performance. CREC Citrus Plant Improvement Table 2. St. Helena Rootstock Survey Trial 2018-19 Valquarius tree size, spacing and HLB status, for trees planted in 2008, sorted by tree height and tree width. Rootstock Tree height* [ft.] Tree width* [ft.] Orange 1804 9.0 11.9 FG 1707 8.7 10.2 Volk 8.5 12.7 Orange 3 [UFR 1] 8.3 9.4 FG 1793 8.2 10.9 Amb+HBJL-2B 8.2 10.5 681G26F4P6 8.2 10.6 White 1805 8.0 11.5 White 4 7.9 9.7 Blue 4 7.8 9.9 681G26F4P2 7.8 9.8 Swingle 7.7 12.0 Cleo 7.7 11.7 Kuharske 7.7 11.0 FG 1733 7.7 10.4 MG-11 7.7 9.5 Orange 4 [UFR 2] 7.7 10.9 Orange 19 [UFR 4] 7.5 10.2 FG 1792 7.5 8.5 Blue 1 7.4 8.9 Purple 2 7.4 8.7 681G26F6P20 7.3 9.9 Cleo+CZO 7.2 9.9 Rough lemon 7.2 9.9

69LTXamF14P37 7.2 9.7 (*) Measured. (**) Calculated using measured width and hypothetical 20 ft. between all rows. (***) Southern Gardens diagnostic lab, PCR analysis of 4-tree composite sample. Actual spacing [trees/acre] Optimum no. trees/acre** HLB diagnosis*** Ct value 12 x 20 [182] 12 x 20 [182] 15 x 25 [116] 9 x 20 [242] 12 x 20 [182] 12 x 20 [182] 12 x 20 [182] 12 x 20 [182] 12 x 20 [182] 9 x 20 [242] 12 x 20 [182] 15 x 25 [116] 15 x 25 [116] 15 x 25 [116] 12 x 20 [182] 12 x 20 [182] 12 x 20 [182] 12 x 20 [182] 12 x 20 [182] 9 x 20 [242] 9 x 20 [242] 12 x 20 [182] 9 x 20 [242] 15 x 25 [116] 12 x 20 [182] 184 213 171 235 202 208 207 192 226 229 225 182 186 198 212 230 203 213 257 248 249 221 221 221 224 No HLB found No HLB found Questionable Questionable No HLB found Questionable HLB positive HLB positive HLB positive HLB positive Questionable HLB positive No HLB found HLB positive HLB positive HLB positive Questionable HLB positive HLB positive HLB positive HLB positive HLB positive No HLB found Questionable HLB positive 32 32 31 30 33 30 30 28 28 28 30 29 32 28 27 29 30 29 27 29 29 27 32 31 28 CREC Citrus Plant Improvement Table 2 (contd). St. Helena Rootstock Survey Trial 2018-19 Valquarius tree size, spacing and HLB status, for trees planted in 2008, sorted by tree height and tree width. Rootstock Tree height* [ft.] Tree width* [ft.] FG 1731 7.2 9.5 Pink 1802 7.2 9.1 Aqua 1803 7.1 9.4 681G26F2P12 7.1 8.4 Chang+50-7 [UFR 6] 7.1

7.2 Orange 13 7.1 9.6 Orange 16 7.0 8.8 FG 1709 7.0 8.4 Orange 15 [UFR 3] 6.9 9.5 Blue 2 6.9 8.7 White 1801 6.7 9.5 FG 1702 6.7 8.5 Green 7 6.7 8.5 Blue 9 6.7 8.4 SO+50-7 6.7 6.9 Blue 3 6.6 7.2 Wgft+50-7 6.5 7.6 Orange 18 6.4 7.0 Chang+Bent 6.4 7.6 SO+CZO 6.3 8.1 Amb+HBJL-1 6.2 8.5 Purple 4 6.2 7.6 Orange 21 5.9 8.7 Orange 14 5.7 8.3 SO+RPxSH99-5 5.7 5.7 (*) Measured. (**) Calculated using measured width and hypothetical 20 ft. between all rows. (***) Southern Gardens diagnostic lab, PCR analysis of 4-tree composite sample. Actual spacing [trees/acre] Optimum no. trees/acre** HLB diagnosis*** Ct value 12 x 20 [182] 12 x 20 [182] 12 x 20 [182] 12 x 20 [182] 9 x 20 [242] 12 x 20 [182] 12 x 20 [182] 12 x 20 [182] 12 x 20 [182] 9 x 20 [242] 12 x 20 [182] 12 x 20 [182] 12 x 20 [182] 9 x 20 [242] 9 x 20 [242] 9 x 20 [242] 12 x 20 [182] 12 x 20 [182] 9 x 20 [242] 12 x 20 [182] 12 x 20 [182] 9 x 20 [242] 12 x 20 [182] 12 x 20 [182] 9 x 20 [242] 230 243 236 261 301 230 248 261 230 250 230 257 259 264 317 301 290 315 288 272 257 289 252 264 391 HLB positive [not sampled] HLB positive Questionable No HLB found HLB positive HLB positive HLB positive HLB positive HLB positive HLB positive HLB positive Questionable HLB positive HLB positive HLB positive No HLB found HLB positive

HLB positive HLB positive No HLB found HLB positive HLB positive HLB positive Questionable 30 27 30 32 29 30 27 28 27 28 29 32 28 28 28 36 28 29 29 32 29 29 29 30 CREC Citrus Plant Improvement Table 3. St. Helena Rootstock Survey Trial 2018-19 Vernia tree size, spacing and HLB status, for trees planted in 2008, sorted by tree height and tree width. Rootstock Orange 1804 Volk Blue 1 MG-11 Purple 2 Orange 19 [UFR 4] Yellow 1800 Cleo+CZO Cleo White 4 [UFR 5] Purple 4 Blue 9 Kuharske Aqua 1803 Wgft+50-7 White 1805 Orange 3 [UFR 1] Swingle Orange 13 Tree height* [ft.] Tree width* [ft.] Actual spacing [trees/acre] Optimum no. trees/acre** HLB diagnosis*** Ct value 9.2 9.1 9.0 8.7 8.6 8.3 8.1 8.1 8.0 8.0 7.9 7.9 7.9 7.9 7.9 7.9 7.7 7.6 7.6 10.5 12.1 10.5 10.7 8.9 9.7 9.5 8.6 10.4 9.8 9.2 8.9 11.6 9.7 8.7 7.7 9.7 10.0 8.9 12 x 20 [182] 15 x 25 [116] 9 x 20 [242] 12 x 20 [182] 9 x 20 [242] 12 x 20 [182] 12 x 20 [182] 9 x 20 [242] 15 x 25 [116] 12 x 20 [182] 9 x 20 [242] 9 x 20 [242] 15 x 25 [116] 12 x 20 [182] 9 x 20 [242] 12 x 20 [182] 12 x 20 [182] 15 x 25 [116] 12 x 20 [182] 208 180 209 206 250 226 230 256 211 224 246 249 190

226 250 282 227 219 246 HLB positive HLB positive HLB positive Questionable HLB positive HLB positive HLB positive HLB positive HLB positive HLB positive Questionable Questionable Questionable No HLB found HLB positive No HLB found HLB positive HLB positive HLB positive 26 28 28 30 29 26 27 29 30 28 30 30 32 32 30 33 30 30 30 9 x 20 [242] 277 No HLB found 32 Chang+50-7 [UFR 6] 7.6 7.9 (*) Measured. (**) Calculated using measured width and hypothetical 20 ft. between all rows. (***) Southern Garden diagnostic lab, PCR analysis of 4-tree composite sample. CREC Citrus Plant Improvement Table 3 (contd). St. Helena Rootstock Survey Trial 2018-19 Vernia tree size, spacing and HLB status, for trees planted in 2008, sorted by tree height and tree width. Tree height* [ft.] Tree width* [ft.] Actual spacing [trees/acre] Optimum trees/acre** HLB diagnosis*** Ct value Orange 2 7.5 11.0 12 x 20 [182] 198 Questionable 30 Blue 4 7.5 7.6 9 x 20 [242] 289 HLB positive 30 Blue 2 7.4 7.8 9 x 20 [242] 279 HLB positive 30 Chang+Bent 7.4 9.3 9 x 20 [242] 240 Questionable 32 Rough lemon 7.3 10.5 15 x 25 [116] 208

Questionable 30 White 1801 7.3 10.0 12 x 20 [182] 218 HLB positive 26 Orange 21 7.3 9.0 12 x 20 [182] 246 Questionable 31 SO+RPxSH99-5 7.3 7.8 9 x 20 [242] 282 HLB positive 28 Blue 3 7.2 7.2 9 x 20 [242] 320 HLB positive 27 Orange 15 [UFR-3] 7.0 9.4 12 x 20 [182] 233 HLB positive 30 Green 7 7.0 9.3 12 x 20 [182] 235 HLB positive 28 Orange 1 7.0 8.7 12 x 20 [182] 256 HLB positive 27 SO+CZO 7.0 8.4 9 x 20 [242] 261 HLB positive 29 Orange 18 6.9 8.4 12 x 20 [182] 261 Questionable 31 Orange 4 [UFR-2] 6.8 8.7 12 x 20 [182] 254 Questionable 31

Orange 14 6.6 8.4 12 x 20 [182] 279 No HLB found 34 Amb+HBJL-2B 6.5 8.0 12 x 20 [182] 273 HLB positive 28 SO+50-7 6.5 7.9 9 x 20 [242] 281 HLB positive 27 Pink 1802 6.4 7.6 12 x 20 [182] 290 HLB positive 29 Rootstock (*) Measured. (**) Calculated using measured width and hypothetical 20 ft. between all rows. (***) Southern Garden diagnostic lab, PCR analysis of 4-tree composite sample. CREC Citrus Plant Improvement Table 4. St. Helena Rootstock Survey Trial 2018-19 Valquarius yield, estimated cumulative yield and PS for trees planted in 2008, sorted by estimated cumulative PS/acre. Rootstock PS/box Boxes/tree Optimum no. trees/acre* Estimated PS/acre [increase 2017-18 to 2018-19, %] Estimated boxes/acre [increase 2017-18 to 2018-19, %] Estimated Cum PS/acre 2010-11 to 2018-19 FG 1731 Orange 3 [UFR 1] Orange 13 White 1805 681G26F4P6 FG 1733 Amb+HBJL-2B Aqua 1803 Cleo+CZO Orange 14 FG 1707 Chang+50-7 [UFR 6] SO+50-7 FG 1793 Purple 2 White 4 MG-11 Blue 9 Kuharske Blue 2 Swingle Orange 15 [UFR 3] Orange 1804 FG 1709 681G26F6P20 6.7 6.5 6.6 6.1 6.2 6.2 6.4 6.2 5.8 6.9 6.8 6.8 6.5 6.2 6.4 6.6 6.0 6.4 6.2 6.7 6.3 5.9 5.9 6.5 6.3 1.1 1.8 2.0 2.9 2.2 1.9

2.3 2.2 1.1 0.9 1.9 1.2 1.0 3.0 1.0 1.4 2.1 1.1 2.8 0.9 1.8 1.7 3.2 1.1 1.6 230 235 230 192 207 212 208 236 221 264 213 301 317 202 249 226 230 264 198 250 182 230 184 261 221 1652 [89] 2699 [113] 2973 [103] 3401 [176] 2858 [62] 2529 [163] 3074 [224] 3146 [160] 1368 [56] 1554 [133] 2808 [73] 2390 [96] 2101 [90] 3711 [178] 1654 [27] 2012 [56] 2836 [110] 1882 [69] 3475 [121] 1479 [-21] 2008 [34] 2225 [65] 3523 [109] 1833 [37] 2265 [91] 247 [56] 417 [101] 451 [73] 559 [146] 463 [46] 410 [109] 483 [177] 511 [125] 234 [51] 227 [102] 412 [46] 350 [81] 325 [72] 601 [160] 259 [15] 307 [33] 477 [69] 293 [58] 561 [95] 221 [-30] 321 [17] 379 [40] 597 [85] 281 [27] 358 [64] 17535 16549 16407 15839 15580 15152 15134 14713 14643 14481 14226 14183 14030 14021 13961 13906 13888 13884 13842 13481 13461 13433 13289 13266 13151 (*) Calculated using measured width and hypothetical 20 ft. between all rows. CREC Citrus Plant Improvement Table 4 (contd). St. Helena Rootstock Survey Trial 2018-19 Valquarius yield, estimated cumulative yield and PS estimates for trees planted in 2008, sorted by estimated cumulative PS/acre Rootstock Amb+HBJL-1 Orange 4 [UFR 2] Pink 1802 FG 1792 Blue 4 Volk SO+CZO 681G26F2P12 681G26F4P2 Orange 16 Purple 4 Blue 1 Green 7 Wgft+50-7

Orange 21 FG 1702 SO+RPxSH99-5 Orange 18 Orange 19 [UFR 4] Chang+Bent Rough lemon Cleo White 1801 Blue 3 69LTXamF14P37 PS/box Boxes/tree Optimum no. trees/acre* Estimated PS/acre increase 2017-18 to 2018-19 [%] Estimated boxes/acre [increase 2017-18 to 2018-19, %] Estimated Cum PS/acre 2010-11 to 2018-19 6.6 6.2 6.4 6.7 5.8 5.8 6.7 6.6 6.1 5.3 6.9 6.6 6.1 6.3 6.1 6.6 6.9 6.3 6.1 6.7 5.9 6.0 6.5 6.9 6.0 1.1 1.8 1.8 1.2 1.8 2.6 0.9 1.3 1.8 1.9 0.6 1.1 1.2 0.9 1.4 1.2 1.1 1.2 1.8 0.3 1.5 1.5 1.6 0.3 1.1 257 203 243 257 229 171 272 261 225 248 289 248 259 290 252 257 391 315 213 288 221 186 230 301 224 1858 [114] 2272 [82] 2832 [71] 1967 [5] 2466 [144] 2539 [84] 1707 [53] 2175 [57] 2428 [176] 2430 [117] 1127 [-6] 1856 [106] 1835 [30] 1725 [55] 2093 [77] 1943 [46] 2912 [105] 2371 [60] 2312 [134] 557 [-39] 1902 [163] 1621 [165] 2345 [233] 677 [-50] 1475 [125] 282 [88] 369 [59] 443 [51] 295 [-9] 423 [136] 441 [47] 256 [27] 328 [35] 396 [130] 460 [106]

164 [-18] 282 [62] 301 [16] 273 [37] 343 [56] 295 [31] 421 [81] 374 [45] 378 [94] 83 [-45] 325 [102] 269 [111] 362 [174] 98 [-59] 246 [101] 13001 12943 12942 12941 12742 12692 12611 12530 12479 12182 12016 11377 11203 11019 10715 10670 10637 10422 10334 10245 9849 9444 9416 9346 8630 (*) Calculated using measured width and hypothetical 20 ft. between all rows. CREC Citrus Plant Improvement Table 5. St. Helena Rootstock Survey Trial 2018-19 Vernia Yield, estimated cumulative yield and PS estimates for trees planted in 2008, sorted by estimated cumulative PS/acre Rootstock PS/box Boxes/tree Optimum no. trees/acre* Estimated PS/acre [increase 2017-18 to 2018-19, %] Estimated boxes/acre [increase 2017-18 to 2018-19, %] Estimated Cum PS/acre 2010-11 to 2018-19 Cleo+CZO Blue 1 Purple 2 Aqua 1803 Chang+Bent Orange 1804 Wgft+50-7 Blue 2 Amb+HBJL-2B MG-11 Purple 4 Orange 19 [UFR 4] Volk Orange 14 Orange 21 Blue 4 Orange 18 White 1805 Chang+50-7 [UFR 6] Yellow 1800 6.9 6.5 6.1 6.6 6.7 6.5 6.7 7.0 6.7 6.8 7.5 6.4 5.6 6.8 6.6 6.5 6.7 6.9 7.3 6.5 1.8 2.5 2.0 2.2 1.2 2.9 1.9 1.1 1.3 2.3 1.1 1.9 3.5 1.1 1.9 1.0 1.5 1.4 1.4 2.4 256 209 250 226 240 208 250 279 273 206 246 226 180

279 246 289 261 282 277 230 3136 [65] 3346 [201] 3026 [29] 3363 [130] 1905 [23] 3906 [147] 3079 [115] 2159 [73] 2369 [144] 3209 [91] 1952 [45] 2773 [150] 3548 [115] 2090 [99] 3099 [101] 1855 [69] 2612 [62] 2776 [95] 2841 [221] 3580 [134] 454 [40] 512 [156] 494 [8] 508 [85] 284 [12] 604 [112] 463 [118] 309 [62] 355 [159] 473 [54] 261 [12] 433 [105] 637 [94] 306 [53] 470 [61] 287 [56] 390 [51] 402 [64] 388 [136] 548 [117] 18128 18051 17565 16844 16601 16464 16356 15995 15820 15719 15145 14762 14525 14390 14131 13919 13863 13778 13665 13477 (*) Calculated using measured width and hypothetical 20 ft. between all rows. CREC Citrus Plant Improvement Table 5 (contd). St. Helena Rootstock Survey Trial 2018-19 Vernia Yield, estimated cumulative yield and PS estimates for trees planted in 2008, sorted by estimated cumulative PS/acre Rootstock PS/box Boxes/tree Optimum no. trees/acre* Estimated PS/acre [increase 2017-18 to 2018-19, %] Estimated boxes/acre [increase 2017-18 to 2018-19, %] Estimated Cum PS/acre 2010-11 to 2018-19 Blue 9 Orange 2 White 4 [UFR 5] Kuharske SO+50-7 Swingle Orange 3 [UFR 1] Blue 3 Green 7 Orange 13 Orange 1 SO+CZO Orange 15 [UFR 3] White 1801 Orange 4 [UFR 2] SO+RPxSH99-5 Cleo Pink 1802 7.1 6.9 6.9 6.9 7.1 6.7 6.8 6.7 7.1 6.9 7.0 7.3 NA 6.6 6.7 7.1 5.6 6.9 1.0 0.9 1.4 2.6 0.8 1.8 1.6 0.9 1.2 1.4

1.5 1.0 1.0 1.4 1.1 0.4 1.7 1.1 249 198 224 190 281 219 227 320 235 246 256 261 233 218 254 282 211 290 1712 [42] 1230 [-1] 2234 [186] 3371 [96] 1567 [57] 2653 [126] 2434 [117] 2020 [168] 1995 [75] 2339 [98] 2607 [178] 1974 [178] NA [NA] 2017 [69] 1901 [105] 796 [-32] 1972 [91] 2101 [130] 240 [31] 179 [-12] 322 [100] 487 [68] 221 [40] 398 [98] 360 [78] 301 [146] 281 [51] 341 [69] 375 [137] 272 [120] 233 [20] 306 [27] 282 [76] 113 [-41] 350 [103] 305 [111] 13445 13403 13135 13080 13015 12640 12617 12584 12362 12204 12189 11216 10596 10302 10100 9417 9102 8089 Rough lemon 6.3 1.2 208 1513 [43] 239 [14] 6801 (*) Calculated using measured width and hypothetical 20 ft. between all rows. CREC Citrus Plant Improvement ge FG 1 3 [U 731 Ora FR 1] W h n ge 1 3 i 681 te 180 G2 6F4 5 P Am FG 1 6 b+H 733 B Aq JL-2B ua Cle 1803 o Ora +CZO nge Cha n g+ FG 14 501 7 [U 707 FR SO 6] +5 FG 0-7 1 Pu 793 rp l Wh e 2 it e MG 4 -1 Bl u 1 Ku e 9 h ar sk

Blu e Ora e n g e S wi 2 n gle 15 Ora [UFR n ge 3] 180 681 FG 1 4 G2 709 Am 6F6P Ora 2 b n ge +HBJ 0 4 [U L-1 Pin FR 2] k1 8 FG 02 179 Bl u 2 e4 Vo l 681 SO+C k ZO G2 681 6F2P G2 12 6 Ora F4P 2 nge Pu 16 rp l e Blu 4 Gre e 1 Wg en 7 ft Ora +50-7 n ge 21 F SO +RP G 170 xSH 2 Or a Ora 99-5 nge n 19 ge 18 Cha [UFR 4 Ro n g+B ] ugh en lem t on Wh Cle o it e 180 69L 1 TXa Blu e mF 14P 3 37 Ora n Cum PS/acre St. Helena Rootstock Survey Trial 9-year estimated cumulative PS/acre for Valquarius trees planted in 2008; 9 seasons, 2010-11 to 2018-19 20000 18000 16000 14000 12000 10000 8000 6000 4000 2000 0 Rootstock CREC Citrus Plant Improvement +C ZO Bl u e1 Pu rp le Aq ua 2 1 Ch an 803 Or g+Be an ge nt W 1804 gft +5 0-7 Am Blu e b+ HB 2 JL2B MG -11 Or P an ge u r pl e 19 [U 4 FR 4] Or Volk an g Or e 14 an

ge 21 Bl u e Or an 4 Ch W ge 1 h an g+ it e 1 8 50 -7 805 [ Ye UFR llo 6] w 18 00 Blu e9 O W hit ran g e4 e [U 2 F Ku R 5] ha rs S O ke +5 0-7 Or S an wi n ge 3 [ gle UF R1 Bl u ] e3 Gr ee n Or an 7 ge 1 Or an 3 ge Or SO 1 an ge + 15 CZO [U F W h it R 3] Or an e1 ge 80 SO 4 [U 1 F +R Px R 2] SH 99 -5 C P i n l eo k Ro ug 1802 hl em on Cle o Cum PS/acre St. Helena Rootstock Survey Trial 9-year estimated cumulative PS/acre for Vernia trees planted in 2008; 9 seasons, 2010-11 to 2018-19 20000 18000 16000 14000 12000 10000 8000 6000 4000 2000 0 Rootstock CREC Citrus Plant Improvement Tree survival, % St. Helena Rootstock Survey Trial 2018-19 survival for Valquarius and Vernia trees planted in 2008 120 100 80 60 40 20 0 Rootstock CREC Citrus Plant Improvement Table 6. St. Helena Rootstock Survey Trial Rootstock parentage and 2018-19 survival of trees planted in 2010, sorted by survival, % Scion

Rootstock Rootstock Parentage Ploidy No. trees planted No. trees survived Survival**, % Valq/Vernia Green 2 [UFR 17] [Nova + HBPummelo] x [sour orange + Carrizo] 4x 17 17 100 Vernia SR+SH-99-11 Sour orange + rangpur, open pollinated 4x 5 5 100 Valq/Vernia 46x31-02-13 [UFR 16] Hirado Buntan (HB) pummelo x Shekwasha 2x 9 9 100 Valq/Vernia 6058-2071-01-02 [Sour orange + rangpur] x [Cleopatra + Argentine trifoliate orange] 4x 24 23 96 Vernia 6058x6056-002 [Sour orange + rangpur] x [sour orange + Palestine sweet lime] 4x 20 18 90 Valq/Vernia Nova+7-2-99-2 Nova + pummelo 7-2-99-2 4x 42 36 86 Valq/Vernia White 1 [Nova + HBPummelo] x [Succari + Argentine trifoliate orange] 4x 24 20 83 Vernia 46x31-02-S3 Hirado Buntan (HB) pummelo x Shekwasha, salinity tolerant 2x 11 9 82 Vernia SO+RPxSH99-4 Sour orange + rangpur, open pollinated 4x 14 11 79 Vernia A-Mac

Tetraploid Macrophylla, Amblycarpa cytoplasm 4x 19 14 74 Valq/Vernia Nova+7-3-99-1 Nova + 7-3-99-1 pummelo 4x 14 10 71 Vernia Amb+Volk Tetraploid Volkameriana, Amblycarpa cytoplasm 4x 42 28 67 Vernia N+HBP-SS-9 Nova + HBPummelo, open pollinated 4x 30 19 63 Vernia 46x31-02-S9 Hirado Buntan (HB) pummelo x Shekwasha, salinity tolerant 2x 12 6 50 Valq/Vernia Amb + 5-1-99-2 C. amblycarpa + 5-1-99-2 pummelo 4x 15 6 40 Valq/Vernia Wmur+HBJL-7 W. Murcott + HBJL-7 pummelo 4x 28 7 25 (*) To be determined (**) Caveat: Many trees of both scions that were lost during the trial were removed early only because of poor condition at planting. Thus, the tree survival results may be somewhat unrepresentative of true performance. CREC Citrus Plant Improvement Table 7. St. Helena Rootstock Survey Trial 2018-19 Valquarius or Vernia tree size, spacing and HLB status, of trees planted in 2010, sorted by tree height and tree width. Scion Valquarius Vernia Vernia Vernia Vernia Vernia Vernia Valquarius Vernia Vernia Vernia Vernia Vernia Vernia Valquarius Vernia Vernia Vernia Vernia Rootstock White 1 46x31-02-13 [UFR-16] 46x31-02-S3 6058x6056-002 Green 2 [UFR-17] SO+RPxSH99-4 46x31-02-S9 6058x2071-01-02 Nova+7-3-99-1 46x31-02-9 A-Mac N+HBP-SS-9 Amb+Volk Nova+7-2-99-2 SO+RPxSH99-4 Amb + 5-1-99-2 6058x2071-01-02 SR+SH-99-11 Wmur+HBJL-7 Tree height* Tree width* [ft.]

[ft.] 8.5 8.3 8.0 7.8 7.7 7.3 7.3 7.1 7.0 7.0 6.8 6.8 6.7 6.6 6.5 6.5 6.5 6.5 6.3 11.5 9.5 8.8 10.2 10.6 8.3 9.8 8.0 10.0 9.2 9.0 8.2 9.1 8.5 7.3 7.3 7.1 6.5 9.3 Optimum trees/acre** HLB diagnosis*** Ct value 190 230 251 215 208 271 224 277 219 240 244 270 240 261 304 301 315 336 236 Questionable HLB positive HLB positive HLB positive Questionable HLB positive HLB positive Questionable HLB positive No HLB found Questionable Questionable Questionable Questionable not sampled HLB positive No HLB found HLB positive HLB positive 30.1 27.4 27.5 29.1 30.3 27.1 28.3 30.9 29.2 32.6 31.0 30.0 31.3 30.2 28.5 34.0 29.7 28.3 (*) Measured (**) Calculated using measured width and hypothetical 20 ft. between all rows (***) Southern Gardens diagnostic lab, PCR analysis of 4-tree composite sample CREC Citrus Plant Improvement Table 8. St. Helena Rootstock Survey Trial 2018-19 Valquarius or Vernia Yield, estimated cumulative yield and PS estimates for trees planted in 2010, sorted by estimated cumulative PS/acre. Scion Valquarius Vernia Vernia Vernia Valquarius Vernia Vernia Vernia Vernia Vernia Vernia Valquarius Vernia Vernia Vernia Vernia Vernia Vernia Vernia Rootstock White 1 46x31-02-13 [UFR 16] 46x31-02-S3 Amb+Volk 6058x2071-01-02

Nova+7-2-99-2 46x31-02-S9 Green 2 [UFR 17] Amb+5-1-99-2 6058x2071-01-02 A-Mac SO+RPxSH99-4 6058x6056-002 Wmur+HBJL-7 46x31-02-9 N+HBP-SS-9 Nova+7-3-99-1 SR+SH-99-11 SO+RPxSH99-4 PS/box Boxes/tree Optimum trees/acre* Estimated PS/acre [increase 2017-18 to 2018-19, %] Estimated boxes/acre [increase 2017-18 to 2018-19, %] Estimated Cum PS/acre 2010-11 to 2018-19 6.2 6.4 6.3 6.5 5.9 6.6 6.9 6.3 6.5 6.4 6.2 6.6 5.9 6.7 6.2 6.8 6.2 6.6 6.1 3.1 2.1 1.8 1.4 1.6 1.5 1.1 1.8 0.7 1.1 1.1 0.7 1.7 1.0 1.1 0.6 1.0 0.2 1.0 190 230 251 240 277 261 224 208 301 315 244 304 215 236 240 270 219 336 304 3630 [240] 3070 [72] 2890 [117] 2187 [93] 2593 [20] 2618 [146] 1664 [-21] 2380 [183] 1272 [16] 2119 [138] 1682 [-9] 1329 [42] 2076 [89] 1506 [14] 1692 [46] 1184 [4] 1280 [25] 446 [-18] 1598 [80] 588 [214] 477 [55] 462 [94] 336 [44] 437 [12] 400 [114] 241 [-31] 377 [134] 196 [2] 332 [123] 270 [-23] 202 [32] 355 [65] 224 [11] 274 [33] 174 [-5] 208 [2] 67 [-15] 262 [83] 9697 9651 9457 9429 8281 8273 8080 7957 7789 7620 7203

7140 7024 6843 6762 5461 4841 4465 4153 (*) Calculated using measured width and hypothetical 20 ft. between all rows CREC Citrus Plant Improvement St. Helena Rootstock Survey Trial Estimated cumulative PS/acre for Valquarius or Vernia trees planted in 2010, 6 seasons, 2013-14 to 2018-19 Cum PS/acre 12000 10000 8000 6000 4000 2000 0 ] ] 9 9 3 k 2 2 2 4 7 9 1 1 4 2 ac 02 Lol -S 2-1 916 -S 917 9-0 9S9-0 0 J 0 2 9 2 M 9 S 9 9 9 1 9 1 V 0 0 0 0 FR FR -9 PHB A56 -2 SH -3 SH b+ -1 11131 1U U : + B H 0 W x x 7 7 5 [ [ x 3 7 3 7 r r m S 6 P + P + 6 x l: 0 0 + u 2 3 +H 6x Ve 8x :4 R+ :A va -2 va -2 +R m +R b 4 n 46 N -1 Va r r 5

S 8 8 o o : : : e 2 O e O 0 : m r r 5 W r 5 0 S V Ve re :S :N :N er :6 r: 1:A l: 60 60 Ve Ve Ve r r G r r V r e 3 : a : : e e e e l r V r V V V V V 6x Ve Va Ve Ve :r 4 Ve te hi 1 Scion: Rootstock CREC Citrus Plant Improvement Tree survival, % St. Helena Rootstock Survey Trial 2018-19 survival for Valquarius or Vernia trees planted in 2010 120 100 80 60 40 20 0 n ee r G 2 ] 17 FR [U +S R S H 1 -1 9 -9 3 -1 2 0 13 x 46 ] 16 FR [U 2 2 2 -0 900 1 9 0 6 -2

105 7 7 6 + 0 8x -2 va 5 8 o 5 N 60 60 W te hi 1 4 3 -S 92 9 0 SH 1x 3 P x +R 46 O S A ac M No v 1 99 -3 7 a+ l Vo + b Am k BP H N+ S -S -9 9 2 7 L9-S J 2 9 B 0 -1 1+H 5 3 r x + u b m 46 W Am Rootstock CREC Citrus Plant Improvement Table 9. St. Helena Rootstock Survey Trial Harrells CRF Fertilizer formulations (utilized in sequence from left to right; of these, 12-3-8, 12-3-9 and 14-3-11 contain TigerSul products). 16-5-10 16.00 5.86 6.86 3.26 13-4-9 13.00 7.47 5.00 0.53 Formulations (%) 12-3-8 12.00 6.94 4.55 0.51 Available Phosphate 5.00 4.00 3.00 3.00 3.00 Soluble Potash 10.00 9.00 8.00 9.00 11.00 Calcium (Ca) 0.00

4.28 4.53 4.53 1.99 Magnesium (Mg) 1.08 1.12 0.98 0.79 1.37 Copper (Cu) 0.06 0.00 0.04 a.n.p 0.04 Iron (Fe) 0.27 0.95 1.10 1.09 0.60 Manganese (Mn) 0.10 0.16 0.92 0.92 1.65 Molybdenum (Mo) 0.01 0.01 0.01 0.01 0.01 Zinc (Zn) 0.06 0.01 0.71 0.71 0.62 a.n.p* a.n.p a.n.p a.n.p 10.15 0.00 0.04 0.05 0.07 0.04 Component Total Nitrogen Nitrate Nitrogen Ammoniacal Nitrogen Urea Nitrogen Sulfur Boron (B) (*) a.n.p - amount not provided 12-3-9 12.00 6.83 4.36 0.81 14-3-11 14.00 5.12 4.50 4.38 CREC Citrus Plant Improvement

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