1 Laboratory Safety Orientation for New Employees USDA

1 Laboratory Safety Orientation for New Employees USDA Agricultural Research Service Pacific West Area Date Updated: November 2007 2 Training Guides Return to the beginning of current module Return to the table of contents Replay the slide audio Reference to the information pamphlet

Module 0: Introduction 3 Training Modules 1. Administrative Information 2. Laboratory Ergonomics 3. Laboratory Hazards and Safety 4. Chemical Exposure 5. Hazard Communication 6. Exposure Control Methods Video Presentation Module 0: Introduction 4 Training Modules

7. Chemical Management 8. Shipping and Transport 9. Hazardous Waste 10. Environmental Management System 11. Emergency Response Challenge Exam Module 0: Introduction 5 Course Objectives Follow USDA safety and security policies Practice good ergonomic work habits Identify laboratory hazards and apply proper protective measures Handle and store chemicals safely Dispose of hazardous waste properly

Know what to do in the event of an emergency on-site Module 0: Introduction 7 Administrative Information Safety and Security Policies Roles and Responsibilities Module 1: Administrative Information 8 Safety and Security Employees must wear ID badges

Do not hold open doors Be mindful of pedestrians in the parking lot Sign in and escort guests at all times Brief guests on important information Module 1: Administrative Information 9 Roles and Responsibilities Employees Properly use protective equipment and clothing Promptly report accidents, injuries and releases to supervisors

Complete required training Supervisors and Managers Ensure employees are properly trained Provide employees access to health and safety documentation Conduct general inspections of laboratories Provide procedures for particularly hazardous operations Module 1: Administrative Information 10 Roles and Responsibilities Senior Management Provide administrative management in program implementation Ensure proper reporting, investigation and follow-up of incidents and accidents

Safety Contact Recommend goals to reduce accidents and incidents Conduct inspections and audits Help managers/supervisors implement policies and procedures Investigate reports of unsafe/unhealthy conditions Module 1: Administrative Information 11 Module Checkpoint Do your part to provide a safe working environment Apply training to communicate effectively with your supervisor or manager

Module 1: Administrative Information 13 Laboratory Ergonomics Musculoskeletal Disorders Ergonomic Pipetting Practices Reporting Injuries Module 2: Laboratory Ergonomics 14 Musculoskeletal Disorder (MSD) Result as a function of: Force Frequency Posture

Overuse of muscles and tendons Lack of recovery time Injuries develop gradually Module 2: Laboratory Ergonomics 15 Ergonomics at the Working Surfaces Avoid excessive reaching with Comfort Zone Elbows at about 90 degrees Nominal reaching with the upper arm

Keep footspaces clear of storage Interrupt repetitive motions with mini-breaks Interrupt static postures by stretching Use proper lifting techniques Module 2: Laboratory Ergonomics 16 Ergonomic Pipetting Practices Keep pipettors in good mechanical condition Service units that become difficult to operate Avoid long durations of pipetting Allow time for mini-breaks

If you feel discomfort when pipetting, inform your supervisor Module 2: Laboratory Ergonomics 17 Reporting Injuries Inform your supervisor and Human Resources department Helpful Injury Information

Location(s) of discomfort Type of discomfort (broad, sharp, tingling, cold, numbing) Recurrence (all the time, only at certain times) History of the injury Tasks Module 2: Laboratory Ergonomics 18 Module Checkpoint

Do your part to reduce your risk to ergonomic injuries Position materials and supplies to reduce reaching Take breaks when working for extended periods of time Keep your equipment well-maintained Report any discomfort from repetitive tasks to your supervisor Module 2: Laboratory Ergonomics 20

Laboratory Hazards and Safety Physical Hazards Protective Measures Module 3: Laboratory Hazards and Safety 21 Physical Hazards Agent Effect Heat and Fire Thermal Burns

Cryogens Frostbite Ultraviolet Exposure Sunburn Explosion Projectile Injury (Chemical Reaction, Cuts/Laceration Compessed Gases) Impact/Bruising Injection Noise Module 3: Laboratory Hazards and Safety Hearing Damage 22

Physical Hazards in the Laboratory Sonicators and Machinery Hearing protection (ear plugs or earmuffs) Ultraviolet Light (direct) Polycarbonate faceshield Cover exposed skin Module 3: Laboratory Hazards and Safety 23 Ultraviolet Radiation Exposure Symptoms Eyes: inflammation, dry or scratchy feeling

Long-term exposure may cause corneal damage Skin: resembles mild sunburn Long-term exposure may possibly cause melanoma Safety Practices Use handheld units in biosafety cabinets PPE is mandatory (especially for face/eyes) Module 3: Laboratory Hazards and Safety 24

Glass Under Pressure Sources of Pressurization: Enclosed environments in glass present an explosion or implosion hazard! Changes in temperature Chemical reaction Direct application of a gas Laboratory Precautions:

Module 3: Laboratory Hazards and Safety Inspect glassware before and after use Note pressure rating Wrap glassware with tape 25 Physical Hazards in the Laboratory Autoclaves Heat and Steam Heat-resistant gloves

Lab coat or apron Cryogens (Liquid Nitrogen) Thermal gloves Lab coat Faceshield Module 3: Laboratory Hazards and Safety 26 Module Interactive In the space provided, list the physical hazards you expect to encounter in your laboratory work. Next to each item, identify the personal protective equipment (PPE) that you will need to safely work with these hazards.

Module 3: Laboratory Hazards and Safety 27 Module Checkpoint Do your part to reduce your exposure to physical hazards in the lab. Identify work that poses a physical hazard. Use the protective equipment available to guard against these hazards. Module 3: Laboratory Hazards and Safety

29 Chemical Exposure Physical and Health Hazards Exposure Routes Toxicity Module 4: Chemical Exposure 30 Physical Hazards Chemical Burns (acids and caustics) Physical Burn (flammable/ combustible) Explosion (chemical reaction

or compressed gases) Asphyxiation (lack of oxygen) Module 4: Chemical Exposure 31 Health Hazards Irritant Allergen/Sensitizer Reproductive/Developmental Toxin Carcinogen Target Organ Effects Chemical Asphyxiant Module 4: Chemical Exposure 32

Routes of Exposure Inhalation Absorption Ingestion Injection Module 4: Chemical Exposure 33 Pinholes compromise the permeability of the glove Fumes from volatile solvents can be breathed in

Working with potentially contaminated gloves A cut from contaminated glass provides direct chemical contact Module 4: Chemical Exposure 34 Module Interactive Avoid wearing gloves to open doors or touch door handles, for the safety of others. 1. What is the rationale for this rule? 2. By which route(s) could contaminated

gloves lead to exposure? Module 4: Chemical Exposure 35 Chemical Toxicity All substances are poisons; there is none which is not a poison. The right dose differentiates a poison from a remedy. -Paracelsus (1493-1541) Acute Chronic Exposure Duration Sudden and severe exposure of high

concentrations into the body Prolonged or repeated exposure over days, months or years. Concentrations vary; may be very little Health Risks Highly symptomatic, reversible health effects with medical treatment Typically non-symptomatic in early stages, irreversible health effects

Examples Cyanide poisoning Concentrated vapors Lead poisoning Breathing dust Module 4: Chemical Exposure 36 Exposure Limits OSHA Permissible Exposure Limit (PEL)

8-hour time weighted exposure for a workers lifetime Based upon chemical toxicity Quantitative assessments are only safe means to determine exposure Smells and Odors are not good indicators of exposure Module 4: Chemical Exposure 37 Module Interactive

Identify whether the condition is the result of acute or chronic exposure. 1. A burning sensation in the eyes from an ammonia splash 2. Numbness of the hands from decades of work with n-hexane 3. Tremors and coma from dermal absorption of an organophosphorous insecticide 4. Hepatitis from daily exposure, over a period of years, to chloroform Module 4: Chemical Exposure 38 Module Checkpoint

Know how to protect yourself from exposure to chemicals in the lab Understand routes of exposure Understand that chemicals can have acute and chronic health effects Module 4: Chemical Exposure 40 Hazard Communication Chemical Hygiene Plan Chemical Labeling Material Safety Data Sheets (MSDS)

Module 5: Hazard Communication 41 The Lab Standard OSHA Regulation 29 CFR 1910.1450 Occupational Exposure to Chemicals in the Laboratory Defines requirements for laboratories: Chemical Hygiene Plan On-the-job training for employees Hazard data of chemicals used (Material Safety Data Sheets)

Module 5: Hazard Communication 42 Chemical Hygiene Plan Found on the EHS website in Tab 6 of the Location Safety Manual

http://webster/EHS/safety.html Defines safe practices for chemical handling Identifies exposure control measures implemented Lists facility policies for treatment of exposure Module 5: Hazard Communication 43 Storage Cabinet and Room Labeling Visible labeling on cabinets containing Acids or corrosive materials Flammable or combustible materials Entrances to areas containing hazards

should identify Hazards present Area supervisor and contact number Module 5: Hazard Communication 44 Hazard Warnings Know the hazards before handling chemicals

Harmful or Toxic Flammable or Combustible Corrosive Oxidizer Peroxidizable Reactive Particularly Hazardous Substances (carcinogens, teratogens, mutagens) Module 5: Hazard Communication Examples of EU pictoral warning symbols on chemical containers. 45

Labeling Hazardous Chemicals Labeling is required on all containers Manufacturers original container Secondary/dispensing containers Waste containers Labeling on USDA secondary containers must include Complete name(s) of chemicals and concentrations Hazardous warning statement Date opened (for peroxidizable chemicals) Labels must be readable at all times; replace worn/degraded labels Check solvent container labels Module 5: Hazard Communication

46 Chemical Labeling Specific hazards are defined in the white portion of the diamond. Module 5: Hazard Communication 47 Hazard Communication Material Safety Data Sheets (MSDS) Hazards and precautions Physical and chemical incompatibilities

Emergency action procedures (First aid, accidental release) Module 5: Hazard Communication 48 Module 5: Hazard Communication 49 Module Interactive The MSDS in this presentation is in your informational pamphlet. Use the MSDS to answer the following questions. 1. Which section has first aid information? 2. List the fire extinguishers recommended for use.

3. What is the NFPA health hazard rating for acetonitrile? 4. Identify the exposure limit for this chemical. 5. What chemicals should not be stored with acetonitrile? Module 5: Hazard Communication 50 MSDS and Informational Sources http://webster/EHS/page9.html Main Building, 3rd Floor

computer room (electronic data files) ToxNet Additional source for chemical hazard data http://toxnet.nlm.nih.gov/ Module 5: Hazard Communication 51 Module Checkpoint

Recognize warnings on chemical containers and take necessary precautions Recognize warnings on doors and cabinets Refer to MSDS to learn important facts about the chemicals, especially hazards and exposure response procedures Module 5: Hazard Communication 53 Exposure Control Methods Engineering Controls Administrative Controls Personal Protective Equipment (PPE)

Module 6: Exposure Control Methods 54 Exposure Controls Control Hierarchy Engineering Controls Administrative Controls Substitution Personal Protective Equipment (PPE) Module 6: Exposure Control Methods 55

Chemical Fume Hood Used for all work with volatiles Optimal air flow conditions: Keep baffles clear Hood sash at recommended setting Adjust baffles settings accordingly Work at least 4-6 inches into the hood Not for chemical storage Keep ignition sources out Module 6: Exposure Control Methods

56 Ventilation Controls Local Exhaust Systems Fume Hoods Balance Enclosures Snorkels Dilution Ventilation Low Toxicity Contaminants Diffuse Contaminant Sources Module 6: Exposure Control Methods 57 Biosafety Cabinet

Type II A 70% air recirculated within enclosure (HEPA filtered) 30% air HEPA filtered and exhausted into the room Type II B2 100% air is HEPA filtered and exhausted to outside For use with volatiles and radionuclides Module 6: Exposure Control Methods 58 Other Engineering Controls Laminar flow, or clean

bench Ventilated balance enclosures Ultraviolet (UV) lamps UV light poses an exposure hazard Use appropriate PPE Module 6: Exposure Control Methods 59 Personal Protective Equipment (PPE) Laboratory Dress Code Face and Eye Protection

Safety Glasses Prescription Glasses Goggles Faceshield Module 6: Exposure Control Methods Body Protection

Lab Coat Apron Shoes Shoe covers Hearing Protection Earplugs Earmuffs 60 Glove Selection Use proper gloves for physical hazards: Friction (work/sharps/broken glass) Thermal (cryogens/heat) Handling chemicals

Latex Nitrile Vinyl Butyl Based on chemical; Use compatibility chart Module 6: Exposure Control Methods 61 Assessment Activity

Note the major hazards in your laboratory and specific control measures implemented in your lab. For example, with each chemical you will use, consider the following: 1. Is there a less hazardous chemical that could be used instead? 2. What engineering controls (e.g., fume hood, glove box, etc.) are needed when working with this chemical? 3. Are there any special procedures or SOPs that need to be followed when working with this chemical? 4. What PPE (e.g., goggles, face shield, gloves (specify type), etc.) must be worn when working with this chemical? Module 6: Exposure Control Methods 62

Module Checkpoint Reduce your risk to exposure by knowing the hazards of chemicals and materials. Hazardous materials have both physical and health exposure risks. A proper combination of administrative and engineering controls helps minimize the risks of exposure. Select PPE based on the hazards of the substances you are using. Know basic emergency response measures.

Module 6: Exposure Control Methods 63 Video Presentations 65 Chemical Management Example of the CIS/Pro Chemical Inventory Software Chemical Ordering and Inventory Chemical Storage Safe Handling Practices Module 7: Chemical Management 66

Chemical Ordering Supervisors approve purchases Supervisor approval for new materials Received chemicals are processed Container(s) barcoded Entered into chemical inventory Module 7: Chemical Management 67

CISPro Inventory Software Log in at http://chemsw Contact your EH&S office for a User Account Module 7: Chemical Management Container-Based Tracking System Linked to Barcode Identification Material Identification Hazard and Container Information Ownership and Location Identification Regulatory

Requirements 68 CISPro Inventory Software Laboratory Users Must Relocate Moved Containers Receiving Cylinders / Stockroom Lab Exchanges Dispose of Empty Containers Keep Accurate Lab Inventories

Use System to Reduce Waste Know Hazard Data and MSDS Utilities Instructions Posted on Locations SHEM SharePoint sites Module 7: Chemical Management 69 CISPro Inventory Software Module 7: Chemical Management 70 CISPro Inventory Software

Module 7: Chemical Management 71 CISPro Inventory Software Module 7: Chemical Management 72 Chemical Storage

Use proper secondary containment Do not store chemicals on floor or in fume hoods Store chemicals below eye level Label stock solutions clearly Keep original labels on all containers Segregate chemicals by hazard never store alphabetically or randomly Use shelving with seismic lips or restraints (for locations at risk to earthquakes) Module 7: Chemical Management 73 Chemical Storage Keep track of time-sensitive chemicals Many solvents and reagents form unstable

peroxides Ethers, Furans Dispose before they form peroxide crystals or dry out Some chemicals must be stored in water Picric acid (keep hydrated) Date each time-sensitive chemical upon opening the container Module 7: Chemical Management 74 Chemical Storage When is it time to dispose of a chemical? Chemicals without an identified use

Outdated chemicals Unknowns* (missing or degraded label) Unsafe or unstable chemicals* Chemicals with unknown users and/or uses * Handle dried-up or crystalized chemicals with extreme care; peroxide crystals are highly unstable and can explode. Module 7: Chemical Management 75 Safe Handling Procedures Wear proper personal protective equipment (PPE) Review the material safety data sheets (MSDS) Check container for special

instructions Know the location of emergency safety equipment Minimize amounts of hazardous materials Module 7: Chemical Management 76 Safe Handling Procedures Know the hazards associated with chemicals in the lab Never smell or taste a chemical Avoid direct contact with any chemical Do not smoke, drink, eat, or apply cosmetics in the lab

Module 7: Chemical Management 77 Safe Handling Procedures Use adequate ventilation Keep metal surfaces grounded Do not remove labels on original containers Label all dispensing containers Chemical name Primary hazards Module 7: Chemical Management 78 Module Interactive

Use the Chemical Compatibility chart in your informational pamphlet to determine if the following chemicals are compatible for storage. 1. 2. 3. 4. 5. Organic solvent & organic pesticide/poison Oxidizer & organic acid Inorganic acid & water reactive Organic poison & inorganic poison Inorganic acid & oxidizer

Module 7: Chemical Management 79 Module Checkpoint Do your part to maintain the safety of chemicals in the laboratory Use the chemical inventory system to manage chemicals Use secondary containment for storage or

transport to reduce the potential for a chemical spill or release Store chemicals only with compatible materials Dispose of obsolete chemicals Module 7: Chemical Management 81 Shipping and Transport Regulatory Agencies and Requirements Packaging and Labeling Module 8: Shipping and Transport 82

Regulatory Agencies Department of Transportation (DOT) DOT regulations apply to any shipment transported by vehicle on public roads (includes all city streets, highways and freeways) International Civil Aviation Organization (ICAO) International Air Transport Association (IATA)

IATA/ICAO are international agencies which are nearly harmonious with DOT and apply to air carriers Module 8: Shipping and Transport 83 Requirements for Shipping Chemicals Classify and identify the material Package material appropriately Label, mark, and seal the package

Complete a Dangerous Goods Declaration When in doubt, ask your Safety contact Module 8: Shipping and Transport 84 DOT Hazard Classes 1. 2. 3. 4. 5. 6. 7. 8.

9. Explosives Compressed Gases Flammable Liquids Flammable Solids Shipping Note Oxidizers Objects shipped with dry ice Toxic Substances must have a DOT Class 9 label on the outer packaging Radioactive Materials Corrosive Materials Miscellaneous Dangerous Goods Module 8: Shipping and Transport

85 Packaging, Labeling and Marking Example: 0.5 liters acetone Plastic inner packaging Absorbent Fiberboard box Acetone

UN1090 LTD QTY 3 Module 8: Shipping and Transport To: From: 86 Packaging, Labeling and Marking

Regulations Safety In case of spill or injury Liability for Damages Customs Homeland Security Module 8: Shipping and Transport 87 Module Checkpoint

Know how to properly prepare a package for shipping Use the appropriate warning and hazard labels Consult with the location Safety contact if you are unsure of the packaging requirements of your shipment Module 8: Shipping and Transport 89 Hazardous Waste Proper Disposal Storage and Labeling

Waste Minimization Module 9: Hazardous Waste 90 Hazardous Waste Chemical waste with these characteristics are considered hazardous waste: Flammability Corrosive materials Reactive materials Toxic substances Specifically listed hazardous substances Module 9: Hazardous Waste 91

Proper Disposal Waste must be properly disposed of Never attempt to dispose waste by: Pouring waste down the drain Diluting with water to compromise criteria East Bay MUD sewer discharge limit of 5.5 pH No strong acids down the drain Evaporating volatiles in fume hoods Disposing in non-regulated trash Module 9: Hazardous Waste 92 Disposing of Containers

A container may be thrown away or recycled if: Empty by *California Law Triple-rinsed Did not contain an extremely hazardous substance (See Tab 4 of Safety Manual) 5 gallons or less in capacity *California empty means nothing remains in the container Module 9: Hazardous Waste 93 Hazardous Waste Satellite Accumulation Areas (SAA) Set the SAA location at or

near the point of generation Keep incompatible wastes segregated Keep containers closed when not in use Storage limit of 6 months Submit request form to have removed or emptied Module 9: Hazardous Waste 94 HAZARDOUS WASTE GENERATOR INFORMATION- USDA, 800 Buchanan Street, Albany, CA 94710

Generator name__________________ Federal Law Prohibits Improper Disposal If found, contact the nearest police, public safety authority or the US-EPA. Contents: Name Percent of total. HAZARD CLASSIFICATION Flammable, Corrosive, Toxic, Reactive Other Halogenated organic solvents are present WASTE FORM Solid, Liquid, Gas

HANDLE WITH CARE Satellite Accumulation Area Date Started = ________ Date Sealed = ________ Module 9: Hazardous Waste Satellite Accumulation Areas Select appropriate containers: Closing glass or plastic bottles for strong acids and causitcs Closing plastic or amber bottles for liquids/solvents Sealable containers for dry waste Requirements: Proper labeling (example shown)

Secondary containment Include the required information 95 1. Chemical waste generated in the labs is collected in a Satellite Accumulation Area (SAA). SAA 3. The waste is prepared for transport by a certified waste hauler to a Treatment/Storage/ Disposal Facility (TSDF). SAA WAA

SAA 2. The waste is collected and transferred to a main Waste Accumulation Area (WAA). Module 9: Hazardous Waste 96 Hazardous Waste Pickup Waste Pickup Information: Pickups are scheduled by Lab Management The waste containers must be prepared prior to pickup Additional requirements apply to

Mixed waste Lecture bottles Unstable or reactive chemicals Unknown waste Remove containers from inventory before disposing Non-compliant waste will not be picked up! Module 9: Hazardous Waste 97 Waste Minimization Minimize chemical purchases Use existing chemical stockroom supplies

Reduce orders to only what you need Practice effective cleanup techniques Participate in your facilitys recycling program Module 9: Hazardous Waste 98 Module Interactive From the list below, identify the necessary information required on the label of a Satellite Accumulation Area (SAA) waste container.

Generator name Color of waste Name(s) of contents Date started Weight of container Form of waste Module 9: Hazardous Waste

Size of container Presence of odors Date sealed NFPA diamond Location of container Date removed 99 Module Checkpoint

Do your part to ensure that waste at the facility is properly managed and disposed Store your hazardous lab waste in a satellite accumulation areas (SAA) Use proper labeling on waste containers Reduce your facilitys waste output by ordering only what will be used Module 9: Hazardous Waste 101 Environmental Management System The EMS Mitigating Environmental Impacts

Module 10: Environmental Management System 102 Environmental Management System (EMS) Goals to reduce environmental impacts Increases awareness Program monitored and audited

Continuous improvement Module 10: Environmental Management System 103 Environmental Fenceline Environmental Impacts Air Runoff & groundwater Soil Impacted Areas

Residential Areas Schools Parklands Streams and Drainage Systems Open Waters Module 10: Environmental Management System 104 Environmental Regulatory Oversight Regulatory Oversight and Enforcement EPA and State/EPA (DTSC)

Regional Water Quality Control Board (RWQCB) Counties Municipal Utilities Districts Air Quality Management Districts Module 10: Environmental Management System 105 EMS Program Objectives Reduce: Energy Consumption Air Pollution Atmospheric Impacts Potential for Facility Contamination and Exposure Risk and Mitigate Impacts of Environmental

Release Potential for Stormwater Contamination Consumption of Natural Resources Generation of Solid Wastes / Increase Recycling Module 10: Environmental Management System 106 Mitigating Environmental Impacts Facility Measures Abatement Control Devices Energy-Efficient Lighting Boiler and Generator Fuel Restrictions and Limits Recycling and E-Waste Laboratory Measures

Chemical Inventory System Hazardous Waste Management Sewer Discharge Limits and Monitoring Module 10: Environmental Management System 107 Mitigating Environmental Impacts Pollution Prevention (P2) Reduction of pollution at the source Minimizing/substituting hazardous materials Efficient use of resources/raw materials Greening the Government Increased recycling and use of

recycled materials Reduce use of environmentally hazardous materials Efficient use of power and fuels Module 10: Environmental Management System 108 Module Interactive Benzene is a flammable, volatile solvent. It is dangerous to the environment and is a known carcinogen. 1. Describe 3 ways this substance can find its way into the environment from a laboratory. 2. Identify a way (for each case) to help prevent this environmental impact from occurring.

Module 10: Environmental Management System 109 Module 10: Environmental Management System 110 Module Checkpoint Do your part to minimize the facilitys impact on the environment Be aware of your facilitys environmental programs

Think about what waste materials you produce can be recycled Module 10: Environmental Management System 112 Emergency Response Emergency Situations Evacuation Procedures Responding to Injuries Module 11: Emergency Response 113 Emergency Support

First-Aid Response Team Spill Response Team O&M Service Desk Location Support Services Center Directors Office After Hours Answering Service Outside Emergency Response Module 11: Emergency Response 114

Emergency Situations What should you do in an emergency? You are expected to `SIN` at a minimum. Safety - ensure your personal safety FIRST! Isolation - alert co-workers to clear out and away from the emergency scene Notification - call the appropriate emergency numbers Module 11: Emergency Response 115

Emergency Situations Building Alarm Earthquake Medical Emergency Civil Disturbance Power Outage Criminal Activity

Industrial Accident Chemical, Biological or Radioactive Spills or Personal Contamination Module 11: Emergency Response 116 Know Your Surroundings Note the locations emergency safety equipment:

Spill Control Station First-Aid Kit Eyewash Station Emergency Shower Fire Extinguisher Fire Pull Station Module 11: Emergency Response 117 Using a Fire Extinguisher

Intended only for small fires Notify co-workers of any fire situation Pull fire alarm (if appropriate) Use PASS Method Pull pin, aim, squeeze, sweep Notify your location safety contact Module 11: Emergency Response 118 Emergency Evacuations Recognize the emergency announcement system and alarms

Familiarizing yourself with the evacuation maps Knowing at least two escape routes Elevators are not an option in an evacuation Module 11: Emergency Response 119 Emergency Evacuations In the event of an evacuation:

Proceed immediately to the assembly area Close corridor doors as you leave Check in with your supervisor Assist visitors and guests Do not leave the assembly area without permission Do not re-enter until the All Clear has been made Module 11: Emergency Response 120 Emergency Evacuations In the event of an evacuation know the location of your assembly area.

Stay off the roadways to allow access for emergency vehicles. Module 11: Emergency Response 121 Module Interactive In the event of an emergency, such as a chemical spill, your immediate response is to SIN. Briefly, describe an action you would take in each step of SINning. Remember: Safety Isolation Notification Module 11: Emergency Response

122 Injuries & Lessons Learned Injury: An incubation flask became stuck to sticky strips on platform of incubator. A lab employee pried flask loose, causing it to shatter and lacerations to hand. Lesson Learned: If applying force to glassware, wear cutresistant gloves. Use minimal force at first to test the resistance of the sticky strip. Module 11: Emergency Response 123

Injuries & Lessons Learned Injury: An employee heated plant growth media in a threaded media bottle (cap loosened / Non Pyrex) then rapidly cooled the bottle under cold water causing bottle to explode resulting in deep incision over left eye. Lesson Learned: Wear eye protection; remove caps or plugs prior to heating media; quick cool glass only if it is tempered (Pyrex) Module 11: Emergency Response

124 Injuries & Lessons Learned Injury: An employee forced a glass pipette into pipetting device. The pipette shattered and the jagged end punctured the palm of hand, requiring several stitches. Lesson Learned: Seek a mechanical aid to load glass pipettes; if there is no mechanical aid, wear punctureresistant gloves for the task Module 11: Emergency Response 125

Injuries & Lessons Learned Injury: An employee received a blistered frostbite burn from contact with liquid nitrogen from a dewar tank Lesson Learned: Wear thermal gloves; Avoid contact with the valves and hoses, and stay clear of the dispensing end of the hose Module 11: Emergency Response 126

Module Checkpoint Do your part to keep the building safe Report unusual occurrences to your supervisor Use the SIN system to respond to emergency situations Keep track of your guests at all times In an evacuation, exit the building immediately and go to your assembly area When an incident occurs, call for help right away

Module 11: Emergency Response 128 Wrap Up Report unsafe conditions, safety concerns, or safety suggestions Submit safety suggestions to supervisor, EH&S Website, or the Location EH&S Office Safety information is available from the EH&S website (http://webster/EHS/safety.html) including:

Safety Committee members and meeting minutes EH&S Contacts Safety policies and chemical hazard information And much more Module 11: Emergency Response 129 Challenge Exam

Print and complete the attached exam: NEO Lab Quiz Submit your completed exam sheet to either your: Location Safety Office Collateral Duty Safety Officer (CDSO) Thank you for taking this training. Challenge Exam

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