The Chemistry of Ethanol Production

The Chemistry of Ethanol Production Sabrina Trupia, Ph.D. NCERC Overview What is NCERC and what do we do Ethanol production Chemistry of front end Chemistry of fermentation Chemistry of Back End

Additional analyses The National Corn-to-Ethanol Research Center (NCERC) MISSION: To facilitate the commercialization of new technologies for producing fuel ethanol more effectively. We are a place for: Demonstration Research Commercial Testing Third Party Validation

Workforce Training R&D NCERC provides under one roof: Analytical Research Lab Fermentation Research Lab Pilot Plant: Dry-Grind & Wet Mill

Workforce Development Training Ethanol production The production of ethanol is among man's earliest ventures into value-added processing. While the basic steps remain the same, the process has been considerably refined in recent years, leading to a very efficient process. Grain Qual DE pH Particle Size pH HPLC Hammer Mill Liquefaction Hopper

Prox. Anal. DE TS % pH Slurry Tank (Cooling) Jet Cooker Fermentation (Heating) FRONT END (190 Proof ) H2O % 9

Ethanol Storage Amino Acids Total P Proximates Ash etc. DDGS BACK END Drum Dryer Distillation System Molecular Sieve 9 (200 Proof ) (Recycled Water)

HPLC Centrifuge TS% Evaporator (Syrup) Whole Stillage Centrifuge (Thin Stillage) ( Wet Cake) TM Analytical tests Front End Grain and Flour

Moisture (oven, moisture balance or NIR) Fat, Protein, Starch (NIR) Particle size Slurry and Liquefaction pH Dextrose Equivalent Analytical Tests Fermentation pH Total Solids

Dissolved Solids Density HPLC 12.000 14.000 10.000 12.000 8.000 DP4+ 6.000 DP2

4.000 Glucose 10.000 Percentage (%) Percentage (%) HPLC 8.000 Ethanol 6.000 Glycerol 4.000 2.000 2.000

0.000 0.000 6 15 21 30 39 45 Fermentation Time (hrs.) 54 62.83 6 15 21

30 39 45 Fermentation Time (hrs.) 54 62.83 Analytical Tests Back End HPLC Proof (water in ethanol) Proximate and specialty analysis of coproducts

GC (ethanol) Additional tests Risk factors Evaluation of feed End-product testing for quality Thank you

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