Three-Point TestCross We want to determine the relative order of & recombination distances among three linked gene loci. Consider three loci E F G whose order is unknown. 2016 by Steven M Carr P: EEFFGG F1 : F2: efg efg efg efg efg efg efg efg EFG EFg EfG
Efg eFG eFg efG efg x EeFfGg eeffgg x eeffgg 370 Parental EF = # Ef + # eF 8 37 = 37 + 95 + 85 + 43 = 260 = 260/1000 = 26 cM FG = # Fg + # fG 95 = 8 + 37 + 43 + 12 = 100 = 100/1000 = 10 cM 85 EG = # Eg + # eG
43 = 8 + 95 + 85 + 12 = 200 = 200/1000 = 20 cM 12 350 Parental 2016 by Steven M Carr E ---------26 cM------------ F -----------20 cM----------G ---10 cM--efg EFg = 8 efg efG = 12 EgF Double recombination rewrite as eGf flips middle locus rewrite as EF = # Ef + # eF + (# EgF + # eGf)(2) = 260 + (8 + 12) (2) = 300 = 300/1000 = 30 cM E --------20cM-------- G ---10cM--- F 2016 by Steven M Carr
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